Cyto-id® green detection reagent
http://cdn035.yun-img.com/static/upload/yjinbio/team/20240502141735_89957.pdf WebThe green dye emits in the green region of the visible light spectrum and is thus not compatible with GFP. b) Flow cytometry application: Make a dye stain solution by diluting 1 μL Green Detection Reagent to 1 mL cell culture medium without Phenol Red Indicator, supplemented with 5% FBS. For
Cyto-id® green detection reagent
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WebIncase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time. ... grow the cells on coverslips after respective treatment incubate them with the dual detection reagent (Cyto‐ID dye and Hoechst 3342 nuclear stain) at 37 ºC for 30 minutes in the ... WebThe CYTO-ID ® Red Tracer Dye fluorescence is independent of pH within normally encountered physiologic ranges and fluorescence intensity per cell is typically unaffected …
WebSep 2, 2024 · When the cells reached 90% confluence, the medium was removed and replaced with 100 µL per well of Microscopy Dual Detection Reagent containing 1 µL of CYTO-ID ® Green Detection Reagent 2 and 1 µL Hoechst 33342 Nuclear Stain mixed in 1 mL neurobasal medium without Phenol Red Indicator, supplemented with 5% FBS. The … WebFeb 14, 2024 · Cyto-ID green detection reagent, consisting of 1 μL Hoechst 33342, 100 μL Cyto-ID reagent per 1 mL cell culture medium supplemented with 10% FBS was used to stained cells. The plate was stored ...
WebAug 6, 2024 · Autophagy analysis was performed by incubating cells with bafilomycin A1 for 30 min at 37 °C prior to treatment with CYTO-ID Green Detection Reagent, and then analyzing fluorescence by flow cytometry using the …
WebJul 16, 2014 · Cyto-ID Green Detection Reagent Kit (Enzo Life Sciences, Farmingdale, NY) detects autophagosomes and autolysosomes as recommended by manufacturer . In …
WebOct 1, 2024 · The autophagy level of haemocytes was detected with CYTO-ID® Autophagy Detection Kit (Enzo, Switzerland) according to the instruction. ... for 3 h, and then washed two times with M-L15 medium. The haemocytes on the slide were incubated with 200 μL CYTO-ID® Green Detection Reagent (diluted 1:500 in M-L15 medium) at 37 °C in dark … shapes year 2WebCYTO-ID® Autophagy Detection Kit 2.0, Enzo Life Sciences Supplier: Enzo Life Sciences A brighter and more photostable, no-transfection, quantitative assay for monitoring … poochie\\u0027s pampered pupsWebApr 21, 2024 · The CYTO-ID green detection reagent was utilized in this study to confirm the findings of western blotting because it selectively stains the autophagic vesicles. Accumulation of autophagosomes was observed in cells treated with TBs-C and HBSS + chloroquine (CQ) . These findings imply that TBs-C therapy can cause the accumulation … poochiful burnleyWebEnzo Life Sciences CYTO-ID Autophagy detection kit (1x50tests) A no-transfection, quantitative assay for monitoring autophagy in live cells. CYTO-ID.The probe is a cationic amphiphilic tracer (CAT) dye that rapidly … poochiful shampooWebThe Cyto-ID™ Autophagy Detection Kit is a member of the CELLestial® product line, reagents and assay kits comprising fluorescent molecular probes that have been … poochiful dog shampooWebFluorescent detection, HTS. Contents: CYTO-ID Green Detection Reagent. Hoechst 33342 Nuclear Stain. Autophagy Inducer (Rapamycin). Chloroquine Control. 10X Assay Buffer Catalog No. 50-103-1813 $558.00 / Each Qty Check Availability Add to cart Provide Content Correction shapes year 8WebDec 22, 2024 · The cell residues were washed twice with the assay buffer, and 100 μl of Dual Detection Reagent (2 μl of Cyto-ID Green Detection Reagent and 1 μl of Hoechst 33,342 Nuclear Stain in 1 mL of cell culture medium) was then dispensed in each well. The cells were protected from light and incubated at 37°C for 30 min. poochigian lawyer fresno