Lamp2b rvg
Tīmeklis2024. gada 11. jūl. · Briefly, Lamp2b was cloned from the C2C12 mouse myoblast cell line with primers containing XhoI and BspEI restriction sites to enable subsequent … Tīmeklis2024. gada 31. janv. · Lamp2b was cloned with cDNA from C2C12 cells and XhoI and BspEI restriction sites were inserted after the signal peptide sequence together with …
Lamp2b rvg
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Tīmeklis2024. gada 21. janv. · 2011年已开发出利用外泌体靶向递送siRNA的新方法，对外泌体表面膜蛋白lamp2b进行改造，并与神经元特异性狂犬病毒糖蛋白 （RVG） 进行融合 … Tīmeklis2024. gada 24. nov. · Lamp2b-HuR融合蛋白功能化的外泌体能够将被RNA结合基序识别的RNA靶标驱动至溶酶体以在受体细胞中降解。 该系统在富含溶酶体的巨噬细胞中 …
http://www.biofeng.com/adjy/zhili/pcDNA%20GNSTM-3-RVG-10-Lamp2b-HA.html Tīmeklis2024. gada 10. janv. · A T7 peptide-decorated exosome (T7-exo) was produced by incorporation of T7 into the exosome membrane as a fusion protein of T7 and Lamp2b. As a control, rabies virus glycoprotein (RVG) peptide targeting brain neuron cells was incorporated into the exosome membrane. AMO-21 was loaded into the exosomes …
Tīmeklis2024. gada 2. maijs · RVG-Lamp2b-Modified Exosomes Specially Target the Injured Region To investigate the potential of RVG-Exos to specially deliver miR-124 to the injured region, we established a focal ischemia model. We performed photothrombosis in the somatosensory cortex ( Figure 2 A). TīmeklisClosed for Inventory Dec 27th - 30th. Sorry for the Inconvenience.
Tīmeklis2024. gada 16. dec. · Abstract. Huntington's disease is an autosomal-dominant neurodegenerative disease caused by CAG expansion in exon 1 of the huntingtin (HTT) gene. Since mutant huntingtin (mHTT) protein is the root cause of Huntington's disease, oligonucleotide-based therapeutic approaches using small interfering RNAs (siRNAs) …
Tīmeklis2024. gada 31. janv. · Expression of MSP-Lamp2b and RVG-Lamp2b was verified with qRT-PCR of dendritic cells transfected with pLamp2b-MSP and pLamp2b-RVG using specific primers for the MSP and RVG sequences (FIG. 2C). As the topology of Lamp2b on exosomes was unknown, a pulldown assay with anti-FLAG beads was designed … cedar point breakers express promotional codeTīmeklis2024. gada 15. nov. · 细胞外小囊泡（sEVsRVG） 的脑部神经特异性靶向是通过将sEVs膜蛋白lamp2b和神经靶向性的狂犬病毒糖蛋白衍生肽（RVG）融合递呈在 细 … cedar point breakers hotel layout roomsTīmeklis2024. gada 20. sept. · 作者分别将含RVG-Lamp2b和T7-Lamp2b质粒转染入293T细胞，分离外泌体后再通过WB验证得到的RVG-exo和T7-exo都带有靶向配体。 随后通 … but they rely ever attain all objectiveTīmeklis2024. gada 25. febr. · c Western blot analysis of Lamp2b, CD63, GM130, and Alix in RVG-Lamp2b-modified exosomes and parental cells. d Expression levels of miR-193b-3p quantified in exosomes in RVG/Exos/scrambled miRNAs (Control) vs RVG/Exos/miR-193b-3p using qRT-PCR analysis. The data were normalized to U6 expression and … cedar point breakers hotel dealsTīmeklis2024. gada 1. dec. · A fusion protein of RVG and Lamp2b was expressed in 293T cells. Since Lamp2b is an exosome membrane-integral protein, RVG-Lamp2b is … but they re entertaining liesTīmeklisSnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of your plasmids, DNA and protein sequences. Annotate features on your plasmids using the curated feature database. Store, search, and share your sequences, files and maps. cedar point breakers promo codeTīmeklis2024. gada 10. janv. · To generate RVG-exo and T7-exo, stable cell lines were prepared by transfection of plasmids encoding RVG-Lamp2b and T7-Lamp2b into 293 T cells (Fig. 1 A).Then, isolation of ligand-decorated exosomes was confirmed by western blotting with an anti-HA-tag antibody because HA-tags were expressed as a fusion … cedarpoint breakers front desk phone