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WebCells were subsequently grown in 75 cm 2 flasks (TPP, Sigma Aldrich, St. Louis, MO), and kept at 37°C in an environment containing 5% CO 2. Cells (H9c2 and LLC-PK1) were … WebThe inside surface treatment by TPP provides an optimal growth surface on the flask base for the most varied matrix-dependent tissue cultures. The untreated side and top inside … normal blood pressure for adults over 70
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